Main Theme and Variations of Phase Contrast Electron Microscopy

Principles of Phase Contrast (Electron) Microscopy

In phase-contrast light and electron microscopy, one exploits the wave properties of photons and electrons respectively. The principles of imaging with waves are the realm of “Fourier Optics”. As a very first experiment (“Gedankenexperiment”), let us think back to days when we – as children – would focus the (parallel) light waves of the (far away) sun on a piece of paper in order to set it ali...

ANALYTICAL REVIEW Phase Contrast Microscopy and Electron Microscopy

By MARCEL BEsSIS T HE STUDY OF BLOOD CELLS by classic-al Isist.ological and hematological technics does usot seem likely to utsdcrgo munch furthser development, ansd for this reasots a special inst.erest in new methods of imsvestigations, including cytochemistry, electron microscopy, and phase contrast microscopy has developed svit.hius recetit years. We shall try to summarize the resunlts obta...

Phase-contrast and electron microscopy of murine strains of Mycoplasma.

Nelson, John B. (The Rockefeller University, New York, N.Y.), and Michael J. Lyons. Phase-contrast and electron microscopy of murine strains of Mycoplasma. J. Bacteriol. 90:1750-1763. 1965.-Two strains of Mycoplasma pulmonis (associated with infectious catarrh) on examination in fluid culture (20% horse serum-bouillon) by phase microscopy were highly pleomorphic, with many bacilliform elements ...

Scanning electron and phase-contrast microscopy of bacterial spores.

The three-dimensional immages of free and intrasporangial spores produced by scanning electron microscopy show surface structures not visible by phase-contrast microscopy. Although fine surface detail is not elucidated by scanning electron microscopy, this technique does afford a definitive picture of the general shape of spores. Spores of Bacillus popilliae, B. lentimorbus, B. thuringiensis, B...

Phase contrast and interference microscopy with the electron microscope.